I'll be speaking at the ABRF (the Association of Biomolecular Research Facilities) Annual Meeting on March 27th, 2017. I'll be in the Trending Topics Track. #conference
I'll be giving one of the plenary talks at the Canadian Microscopy and Cytometry Symposium, along with a Flow Cytometry Workshop. Wednesday, May 10. Montreal, Canada. http://www.cmcs-scmc.ca/html/plenary.html #conference
Conquering Your Conference
Here's a repost of a LinkedIn article I wrote. Earlier this month, I was asked to present a career development talk at CYTO2015, the International Society for the Advancement of Cytometry's (ISAC's) annual conference. Specifically, I was asked to speak to our young investigators (my colleagues in the prestigious ISAC Scholar's program) about how to maximize our experience at the meeting. The presentation was well-received, with some people asking me to distribute or publish
Abseq - A New Era of Unlimited Multiplexing in Cytometry?
Just read a very exciting paper in Nature's Scientific Reports Journal: Basically, the authors use DNA sequences to tag antibodies instead of fluorochromes. Since an unlimited number of unique sequences can be generated, one could - theoretically - multiplex as many antibodies as wished. We tried this a number of years ago -- planning to read out sequences on the 96-plex Fluidigm BioMark platform instead of a sequencer. We weren't successful because our conjugations weren't
Two-second Tip: CD45RA and CD45RO are interchangeable in peripheral blood T-cells.
When designing T-cell panels for use on peripheral blood samples, the CD45 isoforms RA and RO are really great for discriminating naive and memory cells. When coupled with another marker- like CCR7, CD27, or CD57 - you can identify subpopulations of memory cells, like central, transitional, effector. We don't really measure CD45RA and RO in the same panel, though, because they mostly have inverse expression - cells high for CD45RA are low for CD45RO, and vice versa: You c
Two-second Tip: Review panels without NXN plots
When building multicolor panels, it's important to examine every parameter against every other parameter. Why? Compensation, spreading, or fluorescence aggregates in one channel may look like "real" populations in another. By examining every marker against every other marker, you can identify these problems and fix them before you report an artifact. FlowJo has a great tool - the NXN plot for this. The problem, though, is when the panel is large, the NXN plot becomes unwi
Two-second Tip: Presentation-ready Axes for Flow Cytometry Data
Don't present your data like this, for goodness' sake: We can't see the labels for the axes and the gate frequency is obscuring population of interest. Don't bother trying the fix this in PowerPoint, either... you'll waste a bunch of time remaking the axis labels, and - in this case - trying to find the Symbol font in the menu (arrrrrrgh!!!): Instead, use this two-second tip to start with presentation ready graphics straight out of FlowJo (v9, for Mac). Go to the FlowJo men
Some photos of our new lab!
First, the spectacular views around the building (click to view the full-size image): The view from my temporary office (while the lab is renovated): And, finally, the lab space before cleaning and some minor renovations:
Chattopadhyay Lab Takes Bite Out of Big Apple
Delicious! We've (well, really, I've) started the transition to NYU. So far, so good. This is going to be fun! I'll be Associate Professor (Pathology) and Director of a new Applied Immunology Core. We'll be part of the Isaac and Laura Perlmutter Cancer Center, at NYU-Langone Medical Center. Our group will build a cutting-edge research program that integrates high parameter analysis of protein and transcript expression, in order to provide new (and unique) information abou