April 19, 2017

March 19, 2017

March 19, 2017

Please reload

Recent Posts

What to Expect When You're Expecting

September 5, 2017

Please reload

Featured Posts

Two-second Tip: CD45RA and CD45RO are interchangeable in peripheral blood T-cells.

March 19, 2017

When designing T-cell panels for use on peripheral blood samples, the CD45 isoforms RA and RO are really great for discriminating naive and memory cells.  When coupled with another marker-  like CCR7, CD27, or CD57 -  you can identify subpopulations of memory cells, like central, transitional, effector.  We don't really measure CD45RA and RO in the same panel, though, because they mostly have inverse expression - cells high for CD45RA are low for CD45RO, and vice versa:


 You can take advantage of this in panel building.  A couple of months ago, I was building another T-cell differentiation panel, and found that the only CD45RO reagent I hadn't run out of looked terrible (probably because I was scraping the bottom of the vial for reagent).  Luckily, I had a different channel open and a CD45RA antibody, so I substituted that reagent in my next experiment.  Score!


 Much better staining.  Two-second tip: Sub out CD45RA for RO (or vice versa) to help panel building.


Share on Facebook
Share on Twitter
Please reload

Follow Us
Please reload

Search By Tags
Please reload

  • Facebook Basic Square
  • Twitter Basic Square
  • Google+ Basic Square

© 2019.  Pratip K. Chattopadhyay.


This website is intended as a private communication to assess the potential for a scientific partnership, in the same way that one would privately share slides or a written document.

The content herein does not necessarily state or reflect the view or plans of any funding institution or employer; there is no express or implied endorsement.  No information here shall be used for advertising or product endorsement purposes.

The rain in Spain stays mainly in the plain.